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sheather sucrose flotation concentration research 2007

  • Endoparasites in the feces of arctic foxes in a

    A modified double centrifugation Sheathers sucrose flotation technique was performed on a known quantity (13 g) of feces from each sample. All ascarid eggs, observed by light microscopy, were counted and the number per gram of feces recorded.

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  • Detection of Cystoisospora suis in faeces of suckling

    Percoll174; (GE Healthcare) is a density gradient separation medium of low viscosity, low osmolarity and low toxicity. It has been used as flotation solution for C. suis in piglet faeces with good success but it is expensive and can be replaced by the cheaper sugar salt flotation medium. It is, however, most suitable for concentration of oocysts from faeces by sedimentation for further processing of oocysts, e.g. for

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  • Can anyone fix my sucrose flotation protocol for

    Sheathers Sucrose 454 grams sugar, dissolved in 355 ml water. 6 ml formalin to prevent mold growth. Centrifugation is especially necessary when the flotation procedure is performed using a

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  • Annals of Tropical Medicine Parasitology Taylor Francis

    After the fecal material collection, Cryptosporidium oocysts from each sample were concentrated by Sheathers sucrose flotation technique and discontinuous sucrose gradient concentration.

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  • In Vitro Cultivation of Cryptosporidium Species Clinical

    The 1.103 specific gravity sucrose solution contains 300 ml of Sheather's solution, 600 ml of PBS, and 9 ml of Tween 80; the 1.064 specific gravity sucrose solution contains 200 ml of Sheather's solution, 800 ml of PBS, and 9 ml of Tween 80.

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  • Medical Microbiology Lab Series 7 OP examination of

    Sheathers sucrose solution consists of 454 grams sucrose sugar, dissolved in 355 ml water. 6 ml of formalin is added to prevent mold growth. The method is used preferentially for identification of infections with Toxocara sp, Ancylostoma sp., Trichuris sp., Capillaria sp. and Isospora sp.

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  • Evaluation of the accuracy of parasitological techniques

    The accuracy of the parasitological techniques of Willis, Hoffman Pons Janer or Lutz (HPLJ), Sheather and Faust was evaluated in fecal samples from stray cats caught by the Zoonosis Control Center in Goi226;nia, Goi225;s, Brazil.

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  • Diagnosis of Ecto and Endoparasites in Laboratory Rats

    Summary. This article describes various procedures for screening rats and mice to detect endo or ectoparasitism. Several diagnostic assays will be demonstrated, both those suitable for use on live animals and those used after euthanasia of the animal.

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  • Development of a nested polymerase chain reaction assay

    Supernatant was decanted, and the pellet was resus pended in 2 mL of sucrose solution (Sheather's solution without formalin; 3.74M; specific gravity, 1.27). An additional 10 mL of sucrose solution was added, and the sample was centrifuged again for 5 minutes at 200 215; g.

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  • Method for Detection and Enumeration of Cryptosporidium

    Eight concentration and purification methods were evaluated to determine percentages of recovery of Cryptosporidium parvum oocysts from calf feces. The NaCl flotation method generally resulted in the highest percentages of recovery.

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  • Evaluation of the accuracy of parasitological techniques

    the formol ether concentration technique (DE CARLI, 2001; 472 Rezende, These techniques were flotation in a saturated sucrose solution, density 1.2 g/mL (SHEATHER, 1923), spontaneous sedimentation (HOFFMAN et al., 1934; (2007) compared the efficiency of the Willis

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  • Genotyping of Cryptosporidium isolates from human clinical

    Concentration was performed by modified Sheathers sucrose flotation or Materials and methods by overnight decantation in phosphate buffered saline (pH 7.2) at +4176;C. Purified DNAs were stored at 20176;C until Clinical cases analysis.

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  • Development of a nested polymerase chain reaction assay

    Oocysts were isolated by use of the double centrifugation, sucrose flotation technique followed by 5 washes in 1215; PBS solution. 16 Oocysts were purified via size exclusion by use of 104 and 40 m mesh filters to separate E macusaniensis from Eimeria spp and larger fecal debris.

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  • Evaluation of the accuracy of parasitological techniques

    The use of flotation techniques enables the separation of fecal debris from parasitic structures, the use of Sheather solution with a density of 1.2 g/ml promotes the flotation of the eggs which present density ranging from 1.023 to 1.050 (DAVID LINDQUIST, 1982).

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  • Enteric parasitic infection among HIV infected patients

    A cross sectional study, involving 112 HIV sero positive patients was conducted in Tribhuvan University Teaching Hospital, Public Health Research Laboratory, Kathmandu, Nepal from July 2011 to June 2012.

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  • Prevalence, Genetic Characteristics, and Zoonotic

    ABSTRACT. To assess the prevalence and public health significance of rabbit cryptosporidiosis, a total of 1,081 fecal specimens were collected between October 2007 and April 2008 from rabbits on eight farms in five different areas in Henan Province, China, and were examined by microscopy after Sheather's sucrose flotation and modified acid fast staining.

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  • sheather sucrose flotation concentration research 2007

    sheather sucrose flotation concentration research 2007 Can anyone fix my sucrose flotation protocol for ResearchGateI did sucrose flotation for recovery of Cryptosporidium oocyst from water sample. procedure is performed using a viscous solution like Sh

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  • Immunofluorescence detection of Research Online

    Research Online is the open access institutional repository for the University of Wollongong. For further information contact the UOW Library research [email protected] Publication Details Stibbs, H. H. Ongerth, J. E. (1986). Immunofluorescence detection of Cryptosporidium oocysts in fecal smears. Journal of Clinical Microbiology, 24 (4), 517 521.

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  • Joyce S. Knoll, VMD, PhD, DACVP dvm360

    veterinary medicine dec 01, 2007 A 3 month old intact male boxer was presented for a preadoption evaluation at the Foster Hospital for Small Animals at Tufts

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  • Original article MOLECULAR EPIDEMIOLOGY OF

    2007). Moreover, C. parvum is the main Sheather's sucrose flotation method and oocysts were kept at 4 176;C in 2.5% potas sium dichromate till DNA extraction. Oo cysts were washed with 0.025M phos data are mostly uneven with concentration on humans cases only. Moreover, there is

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  • sucrose flotation concentration research 2007

    FL Dorr Oliver Flotation CellsThe Dorr Oliver Flotation Mechanism is the most energy efficient, technically advanced, forced air flotation machine on the market. sheather sucrose flotation concentration research 2007

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  • sucrose flotation concentration research 2007

    sucrose flotation concentration research 2007. Biofuels supplies and suppliers Journey to Forever. New The Biodiesel Bible. by Keith Addison, Journey to Forever. This is the only book that thoroughly covers the entire subject of making your own biodiesel all the information at the Journey to Forever website and very much more.

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  • INVESTIGATION ON CRYPTOSPORIDIUM INFECTIONS IN WILD

    Pathogen examination. The pathogen examination was evaluated by Sheather's sugar flotation technique, 23 and modified acid fast staining. Briefly, saturated sucrose solution for Sheather's sugar flotation technique contains 500 g sucrose, 320 ml distilled water, and 6.7 ml phenol.

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  • Cryptosporidium species detected in calves and cattle in

    Before DNA extraction, Cryptosporidium 100 % identical to sequences in the database (Table 1). oocysts were concentrated by Sheather's sucrose flotation Two isolates of C. andersoni were 100 % identical to C. method (Ma and Soave 1983).

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  • Immunolocation and enzyme activity analysis of

    Immunolocation and enzyme activity analysis of Cryptosporidium parvum enolase. Rongsheng Mi 1, 2, 3 Oocysts from the faeces were concentrated with Sheathers sucrose flotation as previously described by Chen et al. and RNA integrity was checked in 1.0% agarose gels and concentration was tested by NanoDrop 2000C (Thermo Fisher

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  • A simple and rapid technique for concentration of

    fecal films and by zinc sulphate flotation technique.4 Knowledge of Entamoeba histolytica cyst concentration in the environment is important, especially to reduce the risk of consuming the cysts along with untreated waters.

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  • Jundishapur Journal of Microbiology Molecular Study of

    All samples were concentrated by sucrose flotation procedure (Sheathers method, with a specific gravity of 1.21), and thin smears of the concentrated layer of samples were then prepared on glass slides, air dried, and fixed with methanol, and stained by modified Ziehl Neelsen and investigated by

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  • Intestinal coccidian parasitic infections in rural

    Apr 22, 20110183;32;Fecal samples examination of 310 individuals with variable gastro intestinal (GI) disturbances but negative for intestinal parasites or bacterial pathogens, revealed 97 persons positive for infection with coccidian parasites employing modified ZiehlNeelsen (ZN) staining and Sheathers sucrose floatation technique.

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  • Enteric parasitic infection among HIV infected patients

    Teaching Hospital, Public Health Research Laboratory, Kathmandu, Nepal from July 2011 to June 2012. The fecal samples were processed by direct smear technique, in both normal saline solution and 1 % iodine solution as well as modified acid fast staining (Kinyouns method) after formalin ether concentration and Sheathers sucrose flotation for

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  • Study of intestinal parasites among the immunosuppressed

    Concentration techniques[7] Formol ether sedimentation and Sheathers sugar floatation technique were employed for all specimens for the concentration of the parasitic ova and cysts.

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